Fig. 1.
Identification of miRNAs associated with Toll signaling in Drosophila. A: in silico prediction of miRNAs associated with the Toll signaling pathway in Drosophila was performed using TargetScan, miRanda, and PITA software. Eleven putative miRNAs involved in Toll signaling are shown with their target genes (right). Venn diagram indicates miRNAs commonly predicted by all software (created using the online software Draw Venn Diagram). B: miR-317, miR-964, and miR-958 were verified to influence Drosomycin expression using Gal80ts-UAS-miRNA lines. All miRNA-overexpressing flies and control flies were injected with Micrococcus luteus (M. luteus), and Drosomycin expression was analyzed at 24 h postinfection. Data are presented as means ± SD from at least three independent experiments. Statistical significance was assessed by two-tailed Student’s t-test; ***P < 0.001.