Fig. 4.

miR-958 targets Toll and Dif-RA, not Dif-RC, in S2 cells. A: construct used to generate the 3′-UTR reporter vectors for Toll, Dif-RA, and Dif-RC. Mutant vectors were constructed by replacing seed sequence components with the mutant CCGAGA sequence. B-D: the interactions between miR-958 and its predicted target sequences in the 3′-UTRs of Dif-RA (B), Dif-RC (C), and Toll (D) were determined in Drosophila S2 cells. 48 h after transfection, cells were lysed for luciferase assays; firefly luciferase was normalized to Renilla luciferase activity. Data (B-D) are presented as means ± SD from at least three independent experiments. Statistical significance was assessed by two-tailed Student’s t-test; NS, not significant, ***P < 0.001.