Fig. 5.

miR-958 inhibits expression of Toll, Dif, and Drosomycin in vivo. A and B: the mRNA expression levels of Toll (A) and Dif (B) were determined in miR-958-overexpressing flies and controls at 0, 2, 6, 12, 24, and 48 h after M. luteus infection. C: protein levels of Toll, Dif, and Drosomycin were determined by Western blotting at the same times after M. luteus infection. Data (A and B) are presented as means ± SD from at least 3 independent experiments. Statistical significance was assessed by two-tailed Student’s t-test; *P < 0.05.