Fig. 6.
miR-958 sponge restores Toll signaling in miR-958 overexpression flies. A: the levels of miR-958 were examined in control flies (Gal80ts;Tub>GFP), miR-958-overexpressing flies (Gal80ts;Tub>GFP+miR-958), and miR-958 and miR-958 sponge coexpression flies (Gal80ts;Tub>miR-958+miR-958 sponge) before infection. B: mRNA levels of Drosomycin were determined at 48 h postinfection. C: protein levels of Toll and Dif were determined at 6 h and levels of Drosomycin were determined at 48 h after infection with M. luteus. Data (A and B) are presented as means ± SD from at least three independent experiments. Statistical significance was assessed by two-tailed Student’s t-test; NS, not significant, ***P < 0.001.