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. 2017 Mar 4;17:170. doi: 10.1186/s12885-017-3158-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — MiR-155 targets KRAS. a Schematic representation of KRAS 3’ UTR and relative position of the predicted miRNA- binding site. b Western blot analysis of KRAS protein levels in HEK-293 cells transfected with miR-155 or a scrambled oligonucleotide. Actin expression was analyzed as loading control. A representative experiment is shown. c qRT-PCR analyses of KRAS mRNA expression in HEK-293 cells transfected with miR-155 or a scrambled oligonucleotide, normalized with G6PD. qRT-PCR analysis was performed in triplicate and reported values represent the mean ± SD. d Relative luciferase activity in HEK-293 cells transiently transfected with 3’UTR-KRAS and 3’UTR-KRAS mutated in the miR-155 seed sequence along with the miR 155 or with a scrambled oligonuclotide. e Western blot analysis of KRAS protein in MEFs Cbx7^+/+ (WT) and Cbx7^-/- (KO). Gapdh expression was analyzed as loading control. **, P < 0.01 (t test)