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. 2017 Mar 4;36:39. doi: 10.1186/s13046-017-0508-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PIG3 is required for normal mitotic progression. a Exponentially growing PIG3 depleted and control A549 cells were subjected to immunofluorescent analysis using the indicated antibody. The representative images show aberrant mitotic cells with misalignment chromosomes (arrowheads). b Percentages of mitotic cells showing misaligned chromosomes were counted from three independent experiments (^** P < 0.01). c A549 cells were transfected with control siRNA or PIG3 siRNAs for 48 h and stained with an anti-phosphorylated H3 antibody to determine the percentage of mitosis cell population by flow cytometry. d Quantitative analysis of mitotic index. Results were generated from three independent experiments (^** P < 0.01)