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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: SLAS Discov. 2016 Oct 22;22(2):176–186. doi: 10.1177/1087057116674312

Figure 3. HTS results.

Figure 3

A FRET screen of NCC library (727 compounds, 10 μM) to detect modulators of RyR/FKBP/CaM complex was undertaken in duplicate. FRET of donor-labeled FKBP (D-FKBP) by acceptor-labeled CaM (A-CaM) was calculated from donor fluorescence lifetime. Then FRET for each compound (E) was normalized to DMSO control (E0). (A) Normalized FRET from one screen with hit threshold (>3SD of mean) indicated by grey lines. Reproducible hit compounds and their chemical structure are indicated by arrows. (B) Gaussian fit of the E/E0 distribution. (C) Normalized effects (E/E0) of hits identified in two separate screens.