Skip to main content
. Author manuscript; available in PMC: 2017 Mar 4.
Published in final edited form as: Mol Cell. 2015 Feb 26;57(6):1022–1033. doi: 10.1016/j.molcel.2015.01.015

Figure 3. Skp2-mediated polyubiquitination of LKB1 is critical for LKB1 kinase activity.

Figure 3

(A) In vivo ubiquitination assay in HEK293T cells transfected with the indicated plasmids was followed by IB.

(B) LKB1-deficient HeLa cells with stable restoration of the indicated Flag-LKB1 were subjected to IB.

(C) Control- or LKB1-knockdown Hep3B cells with stable transduction of the indicated shRNA non-targeting myc-LKB1 cultured in the absence of glucose for the indicated time points were subjected to IB.

(D) IPs of exogenous Flag-LKB1 by anti-Flag antibody from HEK293T cells transfected with the indicated Flag-LKB1 were subjected to in vitro LKB1 kinase assay followed by IB.

(E) Hep3B cells with stable transduction of vector (Vec) or Skp2 along with the indicated Flag-LKB1 cultured in the presence (+) or absence (−) of glucose were subjected to IB.

S.E., short exposure; L.E., long exposure.

See also Figure S3.