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. Author manuscript; available in PMC: 2017 Mar 4.
Published in final edited form as: Mol Cell. 2015 Feb 26;57(6):1022–1033. doi: 10.1016/j.molcel.2015.01.015

Figure 4. Skp2-mediated polyubiquitination of LKB1 is crucial for maintaining LKB1 complex integrity.

Figure 4

(A) IPs by anti-Flag antibody from cytosolic fractions of HEK293T cells transfected with Xp-STRAD and the indicated Flag-LKB1 were subjected to IB.

(B) IPs by anti-LKB1 antibody from cytosolic fractions of LKB1-deficient HeLa cells with stable reconstitution of the indicated Flag-LKB1 were subjected to IB.

(C) IPs by anti-IgG control or anti-LKB1 antibody from cytosolic fractions of HEK293T cells with control, Skp2, Ubc13 or MO25 knockdown were subjected IB.

(D) IPs by anti-Flag antibody from Hep3B cells with stable transduction of vector or Skp2 along with the indicated Flag-LKB1 were subjected to IB. The ratio of MO25-LKB1 binding is shown.

See also Figure S4.