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. Author manuscript; available in PMC: 2017 Mar 4.
Published in final edited form as: Mol Cell. 2015 Feb 26;57(6):1022–1033. doi: 10.1016/j.molcel.2015.01.015

Figure 5. Oncogenic Ras induces K63-linked polyubiquitination of LKB1 and activation of the LKB1/AMPK signaling via Skp2.

Figure 5

(A–D) In vivo ubiquitination assays in HEK293T cells (A, B and D) or HEK293T cells with control or Skp2 knockdown (C) transfected with the indicated plasmids were followed by IB. The asterisk in (D) indicates non-specific bands (as loading controls), and the arrowhead indicates the bands corresponding to LKB1.

(E) IPs by anti-IgG control or anti-Skp2 antibody from HEK293T cells transfected with vector or H-Ras were subjected to IB.

(F) WT MEFs with stable transduction of vector or the indicated H-Ras cultured in the presence (+) or absence (−) of glucose were subjected to IB.

(G) IPs by anti-IgG control or anti-LKB1 antibody from Hep3B cells with stable transduction of vector or the indicated H-Ras were subjected to IB.

Phosphorylation of Erk1/2 indicates Ras activation.

See also Figure S5.