Fig. 4.

Gene expression profiling in the thoracic spinal cord after EAE. Gene expression levels were evaluated 37 days after EAE symptoms emerged in samples of control (‘sham’) mice (filled squares) and stimulated (‘stim’) mice (filled circles). Expression of the gene of interest was normalized to GAPDH expression and results in the treated group are graphed as percent of the mean of the corresponding control group. Horizontal bars indicate medians for each group. Note the cytokines (lower three graphs) are displayed on logarithmic vertical axes, which are better to show the large range of values in stimulated mice. Mean days of stimulation ranged over groups from 18.4 to 22.8. Groups sizes were MBP stimulated n=7, control n=10; Notch1 stimulated n=8, control n=10; PDGFRα stimulated n=6, control n=9; TNF stimulated n=7, control n=10; IFNγ stimulated n=6, control n=10; IL-1β stimulated n=5, control n=11. Group sizes varied due to occasional problems with the chemical assay. Significant values in the Mann-Whitney test were for MBP U=10.00, 7, 10, p=0.015; for PDGFRα U=6.00, 6, 9, p=0.012; for TNF U=11.00, 7, 10, p=0.018; for IFNγ U=9.00, 6, 10, p=0.023. Significance is indicated on graphs by an asterisk above the column of the stimulated group.