Table 1.
Primers used for endocannabinoid-related genes (CB1, FABP7, FABP5, FAAH, DAGLα) and reference genes (GAPDH, HMBS).
| Gene | Accession No. | Direction | Sequence | Ta (°C) | Product (bp) |
|---|---|---|---|---|---|
| CB1 | NM_001038652.1 | Forward | GGTCTTCTGTGGACTTAGGG | 58.5 | 113 |
| Reverse | CTCCTCTATTCCTTTGTTGCTC | ||||
| FABP7 | XM_002188827.3 | Forward | TGGTAGACAGCCACAACTTC | 61.7 | 237 |
| Reverse | CTCCATCCAGGGTCACAAC | ||||
| FABP5 | NM_001245600.1 | Forward | TTTGGGTAAATGGTGCCTC | 58.3 | 143 |
| Reverse | GACGGTGATTGTGTCCC | ||||
| FAAH | XM_014872201.1 | Forward | AGGTCTGCTCTATGGAGTGC | 59.5 | 109 |
| Reverse | GTCCTCTGCTGCAGGTTTAT | ||||
| DAGLα | XM_014878708.1 | Forward | GTGATTGAGTTCGTGTACGCC | 61.5 | 118 |
| Reverse | CCACCCAGTTACACACCACC | ||||
| GAPDH | NM_204305.1 | Forward | AGCAATGCTTCCTGCACTAC | 57.5 | 121 |
| Reverse | CTGTCTTCTGTGTGGCTGTG | ||||
| HMBS | XM_002187761.1 | Forward | ACCTGCCAACTTCTCTTCCT | 58 | 140 |
| Reverse | GGTTCCAATCACACTCTTTTCA |