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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Fungal Genet Biol. 2017 Jan 9;100:13–21. doi: 10.1016/j.fgb.2017.01.004

Fig. 1.

Fig. 1

A. Diagram of PbpA orthologs and their protein domain structure. Blue: Ataxin 2 like domain. Green: Lsm Associated Domain. Red: PAB1 binding domain. An: A. nidulans. Sc: S. cerevisiae. Schp: S. pombe. Cn: C. neoformans. Hs: H. sapiens. B. PbpA functionally complements a S. cerevisiae PBP1 deletion in a petite negative assay. Colonies represent 10 fold dilutions. BY4741, the ΔPBP1 parental strain, grows normally on both YPD and YPD + 40 µg/ml ethidium bromide, which induces loss of the mitochondrial genome. Lower densities of the ΔPBP1 strain are unable to survive this loss. Addition of either PBP1 or pbpA does not affect growth or survival in a wild type background, but restores normal growth to the ΔPBP1 parent.