Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Fungal Genet Biol. 2017 Jan 9;100:13–21. doi: 10.1016/j.fgb.2017.01.004

Fig. 4.

Fig. 4

Loss of pbpA results in alterations in sexual development and secondary metabolism. A. Phenotypes of strains grown for 3 days at 37°C on GMM. ΔpbpA displays increased pigmentation and altered sporulation patterns. B. pbpA deletion mutants fail to produce ascospores. Phenotypically, no significant differences are seen among among strain under conditions promoting sexual (CHAMPS medium, 5 days growth in dark) or asexual (GMM, 3 days growth in light). C. When spore number is quantified under each condition, ΔpbpA strains display a significant decrease in ascospore number, producing ∼3% WT numbers. D. TLC analysis of organic extracts. Increased intensity of numerous bands, including ST, is seen in the pbpA mutant. E. Localization of StcS-GFP in WT and pbpA mutant backgrounds. F. Penicillin assay quantifying the zone of inhibition produced from culture supernatants against Micrococcus luteus.