Figure 3.

Control point-based affine transformation procedure for co-registration of histological data and multimodal data. (A) Labeled multimodal images and (B) histological images are manually labeled with points that correspond to matching landmarks in each image. These points are used to determine the appropriate transformation that is applied to the histological dataset; (C) overlay of the two co-registered datasets. Orange color demonstrates overlap of the structural features from each dataset, showing accurate registration of the dataset; (D) a magnified view of (C) corresponding to the area shown in the blue box. Scale bar is 200 µm.