Figure 5.

Classification results of rat mammary tissue. (A) Co-registered histology and zoomed region of the tumor boundary; (B) classification map of corresponding regions. Red corresponds to classified tumor, green corresponds to classified muscle, and blue corresponds to classified collagen along the tumor boundary; (C) radial plot showing the mean multimodal signals of each class demonstrating the associated quantitative multimodal signatures; (D) simplified ellipse plot of the three classes [color legend shown in (C)] showing the characteristic statistical properties of the three classes according to the TPEF, SHG, and FLIM features. Scale bar is 200 µm. TPEF, two-photon excitation fluorescence; FLIM, fluorescence lifetime imaging microscopy; SHG, second harmonic generation.