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. Author manuscript; available in PMC: 2017 Mar 5.
Published in final edited form as: ACS Nano. 2016 Mar 15;10(4):4173–4183. doi: 10.1021/acsnano.5b07425

Figure 5.

Figure 5

(a) Schematic diagram of the bifunctional nanoarray surface with a live T-cell. (b) Fluorescence intensity of both streptavidin-647 and UCHT1 Fab′-568 for hexagonal arrays with various spacings. Normalized pY intensity after 5 min stimulation: (c) plot against nanodot spacing, individual cells shown as dots, *** = p < 0.001, ** = p < 0.01 (Wilcoxon rank sum test); positive control, nonspecifically bound Fab′ and ICAM-1 on bare glass (maximum concentration); negative control, PEG passivation background (minimized concentration). (d) Plot against nanodot density, individual experiments shown as dots.