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Dengue virus specific primers and their expected product size.
| Serotype | Primer pair | Size of PCR products (bp) |
|---|---|---|
| First step: RT-PCR | ||
| All serotypes | D1 + D2 | 511 |
| Second step: serotype detection | ||
| DENV-1 | D1 + TS1 | 482 |
| DENV-2 | D1 + TS2 | 119 |
| DENV-3 | D1 + TS3 | 290 |
| DENV-4 | D1 + DEN4 | 392 |
