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Gene expression changes with time in vitro and effect of osteoinduction. (a) Transcripts with the strongest relationship with PD (36 points from 7 donors). (b) Induced gene expression patterns compared to undifferentiated MSCs throughout culture expansion. ∗p < 0.05; ∗∗p < 0.01. (b) Upregulated genes; (c) time/passage variable genes; solid diamond/line undifferentiated MSCs; hollow diamond/dotted line osteodifferentiated MSCs.
