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List of all primers used for MSP and qRT-PCR assay.
| Primer | Sequence (5′ to 3′) | Amplification size (bp) |
|---|---|---|
| Methylation | 204 | |
| Forward | AGAGGTGATCGGTAATTTTTTAGTC | |
| Reverse | CCTATTACACAAACTCAAACTCGTT | |
| Unmethylation | 203 | |
| Forward | GAGGTGATTGGTAATTTTTTAGTTG | |
| Reverse | CCTATTACACAAACTCAAACTCATT | |
| SHISA3 qRT-PCR | 85 | |
| Forward | GTCTACGTCCCCTTTCTCATCG | |
| Reverse | AGGTGCAACAATAAATAGCCACT | |
| GAPDH qRT-PCR | ||
| Forward | CCATGGAGAAGGCTGGGG | 194 |
| Reverse | CAAAGTTGTCATGGATGACC |
