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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Allergy Clin Immunol. 2016 Sep 5;139(4):1293–1301.e4. doi: 10.1016/j.jaci.2016.07.028

Figure 2. The A144E mutation impairs TACI surface expression, but not mRNA expression, in mouse B cells.

Figure 2

A, qRT-PCR analysis of Tnfrsf13b mRNA levels in purified B220+ splenic B cells from TACI+/+ (+/+), TACI+/− (+/−), TACI+/A144E (+/mut), TACIA144E/A144E (mut/mut), and TACI−/− (−/−) mice. The mRNA expression of Tnfrsf13b compared to that of the housekeeping gene Gapdh is shown as a percentage of the ratio in B cells from TACI+/+ WT controls. B and C, Representative FACS analysis (B) and pooled data (C) of the mean fluorescence intensity (MFI) of TACI surface expression on gated B220+ splenic B cells. D and E, Representative FACS analysis (D) and pooled data (E) of intracellular TACI expression in gated B220+ peripheral B cells. MFI data presented are the net value of TACI expression (MFI of TACI minus MFI of isotype control). Columns and bars in A, C and E represent mean ± SEM (n=3–5 mice per group). * p<0.05, ** p<0.01, *** p<0.001 by Student’s t-test n.d.= not detected.