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. 2017 Mar 6;8:103. doi: 10.3389/fimmu.2017.00103

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Copyright © 2017 Duurland, Brown, O’Shaughnessy and Wedderburn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD161⁺ T cells have a proliferative phenotype. (A) Healthy adult peripheral blood mononuclear cells (PBMC) were stimulated with 1 µg/ml αCD3 + 5 µg/ml αCD28 for 4 days and analyzed for cell cycle profile by flow cytometry using FxCycle Violet. Representative histograms and summary graphs indicating percentage of cells in S and G2M phase within CD161⁻ (○) and CD161⁺ () CD3⁺CD4⁺ T cells and Treg (n = 4). (B) PBMC from healthy adults and children were analyzed ex vivo for expression of Ki67. Representative plots and summary graphs showing percentage Ki67⁺ cells within CD161⁻ (○) and CD161⁺ () Tconv and Treg from healthy adults (n = 16) and children (n = 4). (C) Percentage Ki67⁺ within memory cells, defined as CD45RA⁻CD45RO⁺ cells, gated on CD161⁻ (○) and CD161⁺ () Tconv and Treg in healthy adults (n = 14) and children (n = 4). Statistical significance: *P < 0.05, ***P < 0.001.

Inline graphic — CD161⁺ T cells have a proliferative phenotype. (A) Healthy adult peripheral blood mononuclear cells (PBMC) were stimulated with 1 µg/ml αCD3 + 5 µg/ml αCD28 for 4 days and analyzed for cell cycle profile by flow cytometry using FxCycle Violet. Representative histograms and summary graphs indicating percentage of cells in S and G2M phase within CD161⁻ (○) and CD161⁺ () CD3⁺CD4⁺ T cells and Treg (n = 4). (B) PBMC from healthy adults and children were analyzed ex vivo for expression of Ki67. Representative plots and summary graphs showing percentage Ki67⁺ cells within CD161⁻ (○) and CD161⁺ () Tconv and Treg from healthy adults (n = 16) and children (n = 4). (C) Percentage Ki67⁺ within memory cells, defined as CD45RA⁻CD45RO⁺ cells, gated on CD161⁻ (○) and CD161⁺ () Tconv and Treg in healthy adults (n = 14) and children (n = 4). Statistical significance: *P < 0.05, ***P < 0.001.