Table 3.
Extracts tested in the LiaRS assay, in the susceptibility testing assays, and for High Resolution MS analysis.
| Strain | Extract | IC90 WBE/mL | A. fumigatus | LiaRS_Assay | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| aMedium (days) | S. aureus | S. aureus | H. influenzae | E. faecalis | C. albicans | MF_5668 | HB950 | HB953 | ||||
| CL_8260 | EPI_167 | CLB21526 | ATCC_29212 | MY_1055 | ZOI (mm)|Q | ZOI (mm)|Q(#) | ZOI (mm)|Q | LC-MS | ||||
| F-117,410 | F-117,410-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | 0 | 7|C(2) | 7|C | Related to ramoplanin | |
| F-140,177 | F-140,177-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | 0 | 8|C(2) | 8|C | Related to ramoplanin | |
| F-143,676 | F-143,676-C02-C01 | FR23(13) | 0.06 | 0.06 | (−) | 0.06 | (−) | 0 | 9|B(2) | 8|B | Related to ramoplanin | |
| F-143,677 | F-143,677-C02-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | 0 | 8|C(2) | 8|C | Related to ramoplanin | |
| F-144,426 | F-144,426-C03-C01 | GOT(13) | 0.06 | 0.06 | (−) | 0.06 | 0.06 | 11|A | 14A(2) | 14A | Related to ramoplanin | |
| F-146,703 | F-146,703-C02-C01 | GOT(7) | (−) | (−) | (−) | (−) | (−) | 0 | 9|A(3) | 11|A | Related to ramoplanin | |
| F-146,703-C03-C01 | MPG(7) | (−) | (−) | (−) | 0.06 | (−) | 0 | 13|A(3) | 15|A | Related to ramoplanin | ||
| F-149,663 | F-149,663-C01-C01 | CLA(13) | 0.06 | 0.06 | (−) | 0.06 | (−) | 0 | 16|A(3) | 17|A | Related to ramoplanin | |
| F-149,663-C02-C01 | GOT(13) | (−) | 0.06 | (−) | 0.06 | (−) | 0 | 17A(3) | 19A | Related to ramoplanin | ||
| F-149,663-C03-C01 | MPG(13) | (−) | (−) | (−) | (−) | (−) | 0 | 11|A(3) | 11|A | Related to ramoplanin | ||
| F-151,073 | F-151,073-C01-C01 | FR23(13) | 0.06 | 0.06 | (−) | (−) | (−) | 0 | 8|B(2) | 8|B | Related to ramoplanin | |
| F-151,215 | F-151,215-C01-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | 0 | 7|B(2) | 8|B | Related to ramoplanin | |
| F-151,217 | F-151,217-C02-C01 | GOT(13) | (−) | (−) | (−) | (−) | (−) | 0 | 27|D(2) | 26|D | Related to ramoplanin | |
| F-151,222 | F-151,222-C01-C01 | FR23(13) | 0.015 | 0.03 | (−) | 0.015 | (−) | 0 | 16|A(3) | 18|A | A2 | |
| F-151,222-C02-C01 | GOT(13) | (−) | (−) | (−) | (−) | (−) | 0 | 16|A(3) | 15|A | A2 | ||
| F-152,578 | F-152,578-C01-C01 | DNPM(7) | (−) | (−) | (−) | (−) | (−) | 0 | 7|C(2) | 7|C | Related to ramoplanin | |
| F-161,197 | F-161,197-C01-C01 | GOT(13) | (−) | (−) | (−) | (−) | (−) | 27|E | 27|D(2) | 27|D | Related to ramoplanin | |
| F-161,197-C03-C01 | RAM2(13) | (−) | (−) | (−) | (−) | (−) | 0 | 7|E(2) | 7|E | Related to ramoplanin | ||
| F-161,198 | F-161,198-C02-C01 | MPG(13) | 0.06 | 0.06 | (−) | 0.06 | (−) | 0 | 10|A(2) | 10|A | Related to ramoplanin | |
| F-161,203 | F-161,203-C01-C01 | GOT(13) | 0.06 | 0.06 | (−) | (−) | (−) | 0 | 11|A(2) | 10|A | Related to ramoplanin | |
| F-161,233 | F-161,233-C01-C01 | GOT(13) | 0.06 | 0.06 | (−) | 0.03 | (−) | 0 | 14|A(3) | 16|A | Related to ramoplanin | |
| F-161,272 | F-161,272-C01-C01 | GOT(13) | (−) | 0.06 | (−) | 0.06 | (−) | 0 | 8|B(2) | 8|B | Related to ramoplanin | |
| F-162,175 | F-162,175-C03-C01 | MPG(13) | 0.06 | 0.015 | (−) | 0.03 | (−) | 0 | 15|A(3) | 16|A | Related to ramoplanin | |
| F-168,651 | F-168,651-C02-C01 | GOT(13) | 0.06 | 0.03 | (−) | 0.06 | (−) | 0 | 16|A(3) | 18|A | Related to ramoplanin | |
| F-168,651-C03-C01 | MPG(13) | (−) | 0.06 | (−) | 0.03 | (−) | 0 | 15|A(3) | 15|A | Related to ramoplanin | ||
| F-169,254 | F-169,254-C02-C01 | GOT(13) | (−) | (−) | (−) | 0.06 | (−) | 0 | 16|A(3) | 17|A | Related to ramoplanin | |
| F-169,254-C03-C01 | MPG(13) | (−) | (−) | (−) | (−) | (−) | 0 | 11|A(3) | 11|A | Related to ramoplanin | ||
| F-169,257 | F-169,257-C02-C01 | GOT(13) | (−) | (−) | (−) | (−) | (−) | 0 | 12|A(3) | 13|A | Related to ramoplanin | |
| F-169,257-C03-C01 | MPG(13) | 0.06 | 0.06 | (−) | 0.06 | (−) | 0 | 13|A(3) | 13|A | Related to ramoplanin | ||
| F-169,627 | F-169,627-C01-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | 0 | 14|C(2) | 13|C | Related to ramoplanin | |
| F-170,297 | F-170,297-C02-C01 | GOT(13) | 0.06 | 0.06 | (−) | (−) | (−) | 0 | 12|A(2) | 11|A | Related to ramoplanin | |
| F-170,386 | F-170,386-C01-C01 | FR23(13) | (−) | 0.03 | (−) | 0.06 | (−) | 0 | 13|A(3) | 13|A | Related to ramoplanin | |
| F-170,386-C02-C01 | GOT(13) | (−) | (−) | (−) | (−) | (−) | 0 | 17|A(3) | 19|A | Related to ramoplanin | ||
| F-170,386-C03-C01 | MPG(13) | (−) | 0.06 | (−) | 0.03 | (−) | 0 | 14|A(3) | 16|A | Related to ramoplanin | ||
| F-171,173 | F-171,173-C02-C01 | GOT(13) | 0.06 | 0.06 | (−) | 0.06 | (−) | 0 | 9|B(2) | 9B | Related to ramoplanin | |
| F-171,509 | F-171,509-C01-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | 0 | 12|A(3) | 13|A | Related to ramoplanin | |
| F-171,509-C02-C01 | GOT(13) | (−) | (−) | (−) | (−) | (−) | 0 | 11|C(2) | 11|C | Related to ramoplanin | ||
| F-175,357 | F-175,357-C03-C01 | SOTM(13) | 0.0075 | 0.001875 | 0.06 | 0.0075 | (−) | 0 | 13|A(2) | 12A | Related to ramoplanin | |
| F-175,914 | F-175,914-C02-C01 | FR23(13) | (−) | 0.06 | (−) | (−) | (−) | 0 | 7|C(2) | 7|C | Related to ramoplanin | |
| F-175,917 | F-175,917-C02-C01 | FR23(13) | (−) | 0.06 | (−) | (−) | (−) | 0 | 7|C(2) | 7|C | Related to ramoplanin | |
| F-177,328 | F-177,328-C02-C01 | MPG(7) | 0.03 | 0.015 | (−) | 0.03 | (−) | 0 | 17|A(3) | 20|A | Related to ramoplanin | |
| F-177,430 | F-177,430-C02-C01 | MPG(7) | 0.0075 | 0.00375 | (−) | 0.015 | (−) | 0 | 19|A(3) | 21|A | Related to ramoplanin | |
| F-177,720 | F-177,720-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | 0 | 12|A(3) | 13|A | Related to ramoplanin | |
| F-177,720-C02-C01 | FR23(13) | (−) | 0.03 | (−) | 0.06 | (−) | 0 | 14|A(3) | 13|A | A2/A1 | ||
| F-177,770 | F-177,770-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 14|A(3) | 16|A | A2/A1 |
| F-177,770-C02-C01 | FR23(13) | 0.001875 | 0.001875 | 0.06 | 0.00375 | (−) | (−) | 0 | 14|A(2) | 14|A | related to ramoplanin | |
| F-177,777 | F-177,777-C02-C01 | FR23(13) | 0.015 | 0.015 | (−) | 0.015 | (−) | (−) | 0 | 14|A(3) | 14|A | Related to ramoplanin |
| F-178,247 | F-178,247-C02-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 6|B(2) | 6|B | Related to ramoplanin |
| F-178,252 | F-178,252-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 12|A(2) | 13|A | Related to ramoplanin |
| F-178,253 | F-178,253-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 14|E(2) | 14|E | Related to ramoplanin |
| F-178,645 | F-178,645-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 13|A(3) | 14|A | Related to ramoplanin |
| F-178,645-C02-C01 | FR23(13) | 0.03 | 0.03 | (−) | 0.06 | (−) | (−) | 0 | 13|A(3) | 14|A | Related to ramoplanin | |
| F-178,647 | F-178,647-C01-C01 | CLA(13) | 0.06 | 0.015 | (−) | 0.06 | (−) | (−) | 0 | 13|A(3) | 15|A | A2 |
| F-178,647-C02-C01 | FR23(13) | 0.015 | 0.015 | (−) | 0.03 | (−) | (−) | 0 | 16|A(3) | 17|A | A1/A2 | |
| F-179,400 | F-179,400-C01-C01 | CLA(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 10|A(3) | 11|A | Related to ramoplanin |
| F-179,413 | F-179,413-C01-C01 | CLA(13) | (−) | (−) | (−) | 0.06 | (−) | (−) | 0 | 18|A(3) | 19|A | Related to ramoplanin |
| F-179,413-C02-C01 | FR23(13) | (−) | 0.06 | (−) | 0.06 | (−) | (−) | 0 | 15|A(3) | 16|A | Related to ramoplanin | |
| F-179,454 | F-179,454-C02-C01 | FR23(13) | 0.015 | 0.0075 | (−) | 0.03 | (−) | (−) | 0 | 13|A(3) | 13|A | Related to ramoplanin |
| F-182,202 | F-182,202-C03-C01 | MPG(13) | 0.03 | 0.03 | (−) | 0.06 | (−) | (−) | 0 | 11|A(2) | 11|A | Related to ramoplanin |
| F-182,239 | F-182,239-C01-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 12|A(2) | 12|A | Related to ramoplanin |
| F-182,241 | F-182,241-C01-C01 | FR23(13) | (−) | 0.06 | (−) | 0.06 | (−) | (−) | 0 | 13|A(3) | 13|A | Related to ramoplanin |
| F-182,241-C03-C01 | MPG(13) | (−) | 0.06 | (−) | 0.06 | (−) | (−) | 0 | 11|A(3) | 13|A | Related to ramoplanin | |
| F-186,787 | F-186,787-C01-C01 | CLA(7) | (−) | 0.06 | (−) | 0.06 | (−) | (−) | 0 | 6|B(2) | 6|B | Related to ramoplanin |
| F-188,416 | F-188,416-C01-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 9|C(2) | 9|C | Related to ramoplanin |
| F-188,417 | F-188,417-C01-C01 | FR23(13) | 0.03 | 0.015 | (−) | 0.015 | (−) | (−) | 0 | 14|A(3) | 14|A | Related to ramoplanin |
| F-188,442 | F-188,442-C01-C01 | FR23(13) | (−) | (−) | (−) | (−) | (−) | (−) | 0 | 9|C(2) | 9|A | Related to ramoplanin |
ZOI (mm), Halo of inhibition.
Q, Qualifier (A or B-clear, C or D-hazy, E-very hazy).
(#) β-galactosidase intensity (blue ring).
Ramoplanin analogs: A1 MW = 2538; A2 MW = 2552; A3 MW = 2566.
IC90 > 0.06: (−).
a
Media and days of cultivation. Media composition are indicated in Supplementary Data.