Figure 6.

Changes in microglial cells and GFAP expression with age in P23H-1 rats. Photomicrographs from the mid-dorsal retina of representative cross sections of P23H-1 rats of different ages from P10 to P45, showing immunolabeled microglial cells (Iba1⁺, green, A–E), astrocytes and Muller cells (GFAP⁺, red), and DAPI-labeled nuclei (blue, A'–E'). At P15 there are some microglial cells in the INL (arrows, B). At P21 microglial cells migrate toward the ONL and OS layer, there is increased GFAP expression and decreased thickness of the ONL (C,C'), and those events continue until P45 (D,E,D',E'). Scale bar = 100 μm.