Figure 2.

Glycolytic Labeling Kinetics from ¹³C-Isotope Tracing in Mouse Embryonic Explants Shows Increased Glycolytic Flux in the Posterior PSM

(A) Schematic of the experimental setup for kinetic U-¹³C glucose labeling of mouse embryonic PSM explants. Twenty explants from 10.5-dpc mouse embryos were used for measurement at a single time point. Dashed lines indicate the position of cuts to separate PSM into posterior PSM and anterior PSM. LC-MS/MS, liquid chromatography-tandem mass spectrometry.

(B) Time-course fractional ¹³C labeling of glycolytic intermediates after culturing of the explants with 0.5 mM U-¹³C glucose. y Axis in the graphs represents fractional labeling given as a ratio of peak intensities of fully labeled mass isotopomer to the sum of the fully and unlabeled mass isotopomer for a given metabolite. Solid lines indicate posterior PSM while dashed lines indicate anterior PSM. Results are presented as mean ± SD of n = 3 experiments. Student's t test (paired two-tailed distribution) was used to calculate p values. Statistical significance is displayed as ^∗p < 0.05 and ^∗∗p < 0.01.