Figure 5.

Inhibition of Orn Decarboxylation Abrogates Orn Effects in DCs

(A) Expression of Odc1 transcripts in sub-populations of DCs derived from different tissues.

(B) Kinetic analysis of Ido1 transcripts in WT DCs stimulated with Orn (100 μM) in the presence or absence of 1 mM DFMO for different times (indicated). Data are normalized and presented as in Figure 1A. ^∗∗p < 0.01 and ^∗∗∗p < 0.001 (unpaired Student’s t test; Orn- or Orn plus DFMO-treated versus untreated samples).

(C) In vivo suppression of the activity of HY-pulsed WT CD8⁻ DCs in combination with a minority fraction (5%) of the same cells with no conditioning (untreated, unt) or conditioned in vitro with Orn as in Figure 4C in the presence or absence of DFMO (1 mM); analysis of skin reactivity is as in Figure 4C. ^∗∗p < 0.01 and ^∗∗∗p < 0.001 (paired Student’s t test as in Figure 4C). Data are from one experiment representative of three (B and C; means ± SD of triplicates in B and six samples in C; means ± SD of samples indicated in Table S3 for each DC subset in A). Please see also Figures S2 and S3.