Table 1.
Genes selected for quantitative expression analysis
| Class | Gene name | Acronym | Refseq # | Primer catalog number* |
|---|---|---|---|---|
| Immune regulation | Interleukin-1β | IL-1β | NM_000576 | PPH00171C |
| Interleukin-2 | IL-2 | NM_000586 | PPH00172C | |
| Interleukin-6 | IL-6 | NM_000600 | PPH00560C | |
| Interleukin-8 | IL-8 | NM_000584 | PPH00568A | |
| Interleukin-10 | IL-10 | NM_000572 | PPH00572C | |
| Tumour necrosis factor-α | TNF-α | NM_000594 | PPH00341F | |
| Nitric oxide synthase 2, inducible | NOS2 | NM_000625 | PPH00173F | |
| Nuclear factor of κ light polypeptide gene enhancer in B cells 1 | NFKB1 | NM_003998 | PPH00204F | |
| Sensory ion channels | Purinergic receptor P2X, ligand-gated ion channel, 4 | P2RX4 | NM_002560 | PPH00341F |
| Purinergic receptor P2X, ligand-gated ion channel, 5 | P2RX5 | NM_175081 | PPH19418A | |
| Transient receptor potential cation channel, subfamily V, member 1 | TRPV1 | NM_018727 | PPH08086F | |
| Transient receptor potential cation channel, subfamily V, member 4 | TRPV4 | NM_021625 | PPH16107B | |
| Transient receptor potential cation channel, subfamily A, member 1 | TRPA1 | NM_007332 | PPH12389E | |
| Glutamate receptor, ionotropic, kainate 2 | GRIK2 | NM_021956 | PPH01863A | |
| Glutamate receptor, ionotropic, N-methyl d-aspartate 1 | GRIN1 | NM_007327 | PPH01823F | |
| Serotonin receptor | 5-hydroxytryptamine (serotonin) receptor 1A | HTR1A | NM_000524 | PPH02530E |
| 5-hydroxytryptamine (serotonin) receptor 2A | HTR2A | NM_000621 | PPH01861G | |
| Adrenergic receptors | Adrenergic β-1 receptor | ADRB1 | NM_000684 | PPH02091B |
| Adrenergic β-2 receptor | ADRB2 | NM_000024 | PPH01856E | |
| Catechol-O-methyltransferase | COMT | NM_000754 | PPH01584B | |
| Substance P receptor | Tachykinin receptor 1 | TACR1 | NM_001058 | PPH01825A |
| Nerve growth factor | Brain-derived neurotrophic factor | BDNF | NM_001709 | PPH00569F |
| Antioxidative enzyme | Catalase | CAT | NM_001752 | PPH00420B |
| Sphingosine-1-phosphate pathway | N-acylsphingosine amidohydrolase (acid ceramidase) 1 | ASAH1 | NM_004315 | PPH02492F |
| Sphingosine kinase 1 | SPHK1 | NM_021972 | PPH02491A | |
| Sphingosine-1-phosphate lyase 1 | SGPL1 | NM_003901 | PPH13925A |
*Qiagen catalogue number for primers optimised and validated for human RT² qPCR Primer Assay (Qiagen, Valencia, California, USA).