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. 2017 Feb 8;198(6):2269–2285. doi: 10.4049/jimmunol.1600610

FIGURE 2.

FIGURE 2.

Loss of Twist1 in COL1A2+ cells is associated with enhanced accumulation of T cells following bleomycin injury. (A–E) BAL was processed for flow cytometry for markers of neutrophils, macrophages, and T and B cells. For these experiments, n = 5 per uninjured condition and n = 11–12 for injured conditions. BAL was collected at 14 d after injury. (A) Dot plots of uninjured and bleomycin-injured animals for neutrophils, macrophages, T cells, and B cells. Quantification of (B) Ly6G, (C) CD68 (*p = 0.006, uninjured Twist1 WT versus uninjured Twist1 FL and **p < 0.025 by ANOVA, uninjured plus Twist1 FL versus bleomycin plus Twist1 FL), (D) CD3 (*p = 0.0021, bleomycin plus Twist1 WT versus bleomycin plus Twist1 FL), and (E) B220 (*p = 0.03, uninjured plus Twist1 WT versus uninjured plus Twist1 FL) is shown.