Table I. Putative E-box motifs of CXCL12 and RelB gene.
Gene | Location | E-box Motif | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | PCR Product Size (bp) |
---|---|---|---|---|---|
RelB | −1718 to −1713 | E1 | CACATTTGTATATAGCACCCACATG | GAGAAAACTGAAACCCGGCA | 123 |
−1701 to −1696 | E2 | ||||
−1699 to −1694 | E3 | ||||
−1598 to −1593 | E4 | GCCGGGTTTCAGTTTTCTCA | GTGACAGAGTGAGACCTTGTC | 128 | |
−1558 to −1553 | E5 | ||||
−1389 to −1384 | E6 | CCTCGGCCTCCCAAAGTATT | TCTCAGACCACAGATGCAGG | 114 | |
−1318 to −1313 | E7 | ||||
−1222 to −1217 | E8 | TCAGGAATATGAGAGGCTTAGGA | CCTCCGTATACATCCTGAGCT | 155 | |
−1173 to −1168 | E9 | ||||
−472 to −467 | E10 | ATGGATGGCAGGTGTAGAGC | TCTGGACGAGACAACTGAGG | 177 | |
−320 to −315 | E11 | ||||
−227 to −222 | E12 | TGCCCAACCCCTCCTGAG | GACGTCACGCCTGGGGAA | 101 | |
CXCL12 | −1894 to −1889 | E1 | CTTTCAGGCTTCTGGGACAG | GTCCGCGGGAAATCTACAC | 201 |
−1886 to −1881 | E2 | ||||
−1700 to −1795 | E3 | CCTCCCGGGTTTCATCAG | ACTGAAGGCAGTGAGGTCCA | 194 | |
−1046 to −1041 | E4 | GCTGGGAAGGACACAGAGAG | TGGGTCCAGAAAACTCTTCC | 208 | |
−517 to −512 | E5 | ACTGCAAAGACGGGTCTCAT | GATGGCGGGAACTGAATG | 197 |