Table V. Predicted upstream regulators of Twist1-low (tertile 1) versus Twist1-high (tertile 3).

Upstream Regulator	Molecule Type	Predicted Activation State	Activation z-Score	−Log (p Value of Overlap)	Target Molecules in Dataset
IFNG	Cytokine	Activated	2.431	12.66	ACTB, ADGRG2, AGT, ALDH1A3, AVPR1A, B2M, BATF2, BST2, BTN3A1, C1R, CASP1, CCL11, CD2, CHST7, CHST8, CLIC5, CX3CR1, CXCL11, CXCL9, FHL2, GBP1, GBP4, GCH1, GJB2, GLIS1, GRM8, HIF1A, HMGCR, IDO1, IFI44L, IFIH1, IGF1, IGFBP4, IL23R, ITGAL, KLRC4-KLRK1/KLRK1, LOX, MMP11, MMP3, OAS2, OAS3, PCSK1, PLA2G2A, PRDX2, PSMB9, SCG5, SMAD1, TACR2, TBX21, TIMP1, TLR3, TLR7, TMEM158, TNFAIP6, TNFSF11, TRPC4, WARS
TNF	Cytokine		0.767	10.34	ACTB, ADRB2, AGT, ALDH1A3, APC, AVPR1A, B2M, BDKRB1, BDKRB2, BST2, CASP1, CCL11, COL15A1, COL3A1, CX3CR1, CXCL11, CXCL9, FERMT1, FIGF, FST, FZD5, GADD45A, GBP1, GCH1, HIF1A, HMGCR, IDO1, IFIH1, IGF1, IGFBP4, IGFBP5, IGFBP6, ITGAL, LOX, MMP3, NGFR, NME1, NPPA, NUMB, OAS2, OAS3, OSMR, PLA2G2A, PPAP2A, PSMB9, RBPMS, SERPINF1, SLC2A4, SMAD1, TBX21, TDRD7, TGFB3, TIMP1, TLR3, TLR7, TNC, TNFAIP6, TNFSF11, TPST1, VIP
LPS	Chemical drug		−0.073	9.05	ADRB2, AGT, AK4, AVPR1A, BDKRB1, CASP1, CCL11, CD160, CHST7, COL3A1, COX4I1, CX3CR1, CXCL11, CXCL9, DIO2, DRD5, EHHADH, FBLN2, FST, GADD45A, GBP1, GBP4, GCH1, GJB2, HIF1A, HMGCR, IDO1, IFI44L, IFIH1, IGF1, IGFBP4, IGFBP5, IL23R, ITGAL, LGALS1, MMP3, NGFR, NPPA, OAS2, OAS3, OSMR, PLA2G2A, PSMB9, RNF19A, RXRG, SERPINF1, SLC39A14, TBX21, TIMP1, TLR3, TLR7, TNC, TNFAIP6, TNFSF11, TP53BP2, TPBG,VCAN, VIP, WARS
IL1B	Cytokine		0.178	9.04	ADRB2, AVPR1A, BDKRB1, C1R, CCL11, CX3CR1, CXCL11, CXCL9, FST, GADD45A, GBP1, GCH1, GYS1, HIF1A, IDO1,IGF1, IGFBP4, IGFBP5, IGFBP6, IL23R, LOX, MMP11, MMP3, NPPA, OAS2, OSMR, PCSK1, PLA2G2A, PSMB9, SLC2A4, SLC6A4, TBX21, TGFB3, TIMP1, TLR3, TLR7, TNFAIP6, TNFSF11, VCAN
STAT3	Transcription regulator	Inhibited	−2.600	8.85	AGT, BOC, BST2, CASP1, CCL11, COL3A1, CXCL9, FST, GADD45A, HIF1A, IFIH1, IGFBP5, IL23R, KLRC4-KLRK1/KLRK1, MMP3, NPPA, OAS2, OAS3, PCSK1, PLA2G2A, PSMB9, TBX21, TIMP1, TLR3, TNFSF11, VCAN, VIP, WARS
TGFB1	Growth factor	Inhibited	−3.305	8.77	ACKR1, ADGRG2, B2M, CASP1, CCL11, CHST8, COL3A1, CPXM1, CTNND1, CX3CR1, CYP21A2, ECM1, FBLN2, FERMT1, GADD45A, GBP1, GDF6, GJB2, GREM1, HIF1A, IFIH1, IGF1, IGFBP4, IGFBP5, IGFBP6, IL23R, ITGA7, ITGAL, KDELR2, KLRC4-KLRK1/KLRK1, KLRD1, KRT10, LOX, LTBP1, MMP11, MMP3, MYO1C, NME1, NPPA, OSR2, PPP2R5A, RBPMS, RHOD, SCG5, SLC39A14, SLN, TBX21, TGFB3, TIMP1, TNC, TNFAIP6, TNFSF11, TUBB3, UCK2, USP25, VCAN, VIP, XPNPEP2
SB203580	Chemical, kinase inhibitor		0.741	8.27	ADAMTS9, ADRB2, AGT, BDKRB1, BST2, CCL11, COL3A1, CX3CR1, CXCL11, CXCL9, GADD45A, GPI, HIF1A, IDO1, LMO2, MMP3, NPPA, TGFB3, TIMP1, TLR3, TNFAIP6, TNFSF11, ZFYVE26
IL6	Cytokine		−1.288	7.97	AGT, BST2, CASP1, CCL11, COL3A1, COX4I1, CX3CR1, GADD45A, GREM1, HIF1A, IDO1, IGF1, IGFBP4, IGFBP5, IGFBP6, IL23R, KLRD1, LGALS1, MMP3, PLA2G2A, PSMB9, PTP4A3, SLC2A4, SLC39A14, SMOX, TIMP1, TLR3, TLR7, TNC, TNFSF11, VIP, WARS, XPNPEP2
Estrogen	Chemical drug		−0.763	7.96	AGT, COL14A1, COL15A1, COL3A1, GJB2, HIF1A, HMGCR, IDO1, IGF1, IGFBP4, IGFBP5, IGFBP6, LGALS1, MTA3, NGFR, NME1, SLC6A4, TBX21, TGFB3, TNFSF11
IFNL1	Cytokine	Activated	3.293	7.76	BST2, CXCL11, CXCL9, DDX60, GBP1, IFI44L, IFIH1, OAS2, OAS3, PSMB9, TDRD7
Tretinoin	Chemical, endogenous mammalian		0.159	7.51	AGT, ALDH1A3, CASP1, COL3A1, CRABP2, CX3CR1, CYP21A2, DAPK2, DDX60, DIO2, FERMT1, FST, GBP4, GIMAP2, GREM1, IFI44L, IFIH1, IGF1, IGFBP4, IGFBP5, IGFBP6, IL23R, ITGAL, LGALS1, MMP11, MMP3, MYO1C, NPPA, NUMB, OAS2, OAS3, PRDX2, PRKCH, PSMB9, PTP4A3, RTKN2, RXRG, SLC2A4, SMOX, TGFB3, TIMP1, TLR3, TNC, TNFAIP6, TNFSF11, TRPC4, TUBB3, UBA7, VIP, YPEL1
Rosiglitazone	Chemical drug		0.836	7.48	AGT, APOL3, CCL11, COX4I1, CXCL11, CXCL9, DIO2, GBP1, GPR65, IGF1, IGFBP5, IGFBP6, ITGAL, KLF11, LOX, NPPA, OAS2, OAS3, SERPINF1, SLC2A4, TBX21, TGFB3, TIMP1, TNC
IL1RN	Cytokine	Inhibited	−2.514	7.32	BDKRB1, BTN3A1, CXCL11, GBP1, IFI44L, IFIH1, IGF1, IGFBP4, IGFBP5, OAS2, OAS3, SLC6A4, TIMP1
STAT1	Transcription regulator	Activated	2.169	7.31	AGT, ALDH1A3, B2M, BATF2, CASP1, CLIC5, CXCL11, CXCL9, GBP1, HIF1A, IDO1, IGF1, NPPA, OAS2, PSMB9, TBX21, TLR3, TP53BP2, WARS
IL1	Group		−0.269	7.29	ADRB2, CASP1, CXCL11, CXCL9, FST, FZD5, HIF1A, HMGCR, IDO1, IGF1, LOX, MMP3, NGFR, PLA2G2A, TGFB3, TIMP1, TLR7, TNC, TNFAIP6, TNFSF11, VCAN