Figure 1.

DATS inhibits viability and reduces fibrotic protein expression associated with H₂S in HSCs. (a) LDH release assay. Significance: ^∗∗P < 0.01 versus DMSO and ^∗∗∗P < 0.001 versus DMSO. (b) MTT assay. Significance: ^#P < 0.05 versus DMSO, ^∗P < 0.05 versus DMSO + H₂O₂, and ^∗∗P < 0.01 versus DMSO + H₂O₂. (c) Western blot analyses of profibrotic marker proteins. (d) Morphology evaluation with light microscope (200x magnification). (e) ELISA for H₂S concentration. Significance: ^∗P < 0.05 versus DMSO, ^∗∗P < 0.01 versus DMSO, and ^#P < 0.05 versus DATS 10 μM. (f) Western blot analyses of profibrotic marker proteins.