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. 2017 Jan 25;7(3):397–404. doi: 10.1002/2211-5463.12189

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© 2016 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Phosphorylation of mitotic CTCF. (A) Analysis by phos‐tag SDS/PAGE. Whole cell lysates were separated on a 7.5% SDS/PAGE (upper panel) and a 5% SDS/PAGE containing 20 μm phos‐tag (lower panel), followed by western blotting with anti‐CTCF antibody. (B) Lambda phosphatase treatment of mitotic CTCF. Cell lysates prepared from asynchronous cells (lane 1) or mitotic‐arrested cells (lane 2) were subjected to immunoprecipitation assays using anti‐CTCF antibody. The immunoprecipitated CTCF was incubated in the absence or presence of lambda protein phosphatase and subjected to phos‐tag SDS/PAGE as described above.