Figure 3.

PDGFR signaling is not affected by SCD knockdown or inactivation. Human fibroblasts were infected with lentiviral particles that express either shSCD1 or control shRNA (scramble) or were treated with the SCD inhibitor (20 μm) or vehicle (DMSO) for 24 h in the absence of serum. Cells were treated with PDGF‐BB (25 ng/mL) for 15 min and proteins were extracted. Western blot experiments were performed using (A) anti‐phospho‐tyrosine (pY99), anti‐PDGFRβ, and (B) anti‐phospho‐PLCγ, anti‐phospho‐AKT, anti‐phospho‐STAT3, or anti‐β‐actin antibodies. Similar results were obtained after up to 24 h of stimulation with PDGF‐BB (data not shown).