Table 1.
Primer sequences used for genotyping the SNP markers in pigs
| SNP marker | Primer sequence (5′ to 3′) | Size (bp) | Tm (°C) | Restriction enzyme |
|---|---|---|---|---|
| SSC7 g.4937240C>G | F: TGTCTCTCCTCTTGCCCATC R: CAGTCAAGCCTCTTCTTGTC |
286 | 58 | BsuRI |
| SSC8 g.47338181G>A | F: CTGGCATCATCACCCTAAAC R: CCACACCAGTCTTGTGAACT |
288 | 60 | TaqI |
| SSC9 g.5496647_5496662insdel | F: GCTATGGCTTTCCCTTCTGC R: AGCTTCAGAGCGTCCACGAA |
216/200 | 58 | 16-bp Ins/Del |
| SSC10 g.71225134G>A | F: GCCACCAACCGAGACTACAA R: CTACACGCGGGACAAACAAT |
365 | 58 | RsaI |
| SSC17 g.61976696G>T | F: CTTTCTGGGTGTTCCCTCCA R: CAGGTAACAGACTGGCAGAG |
384 | 58 | Hin6I |
SNP, single nucleotide polymorphism.