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. 2016 Aug 4;30(3):347–354. doi: 10.5713/ajas.16.0372

Figure 5.

Figure 5

Effect of Se yeast supplementation on the plasma P4 concentrations (ng/mL) of all CL that formed during the first 100 postpartum days (A), CL that developed after the third ovulation with estrus (B), CL that developed after ovulation with estrus between 60 to 80 days after calving (C). Blood sampling from the jugular vein was carried out in the morning every two days. Plasma P4 concentrations were measured using an enzyme immunoassay kit after diethylether extraction. All assays were performed in duplicate. Data are presented as mean±standard error of the mean values (−Se: n = 43, +Se: n = 45) in A, (−Se: n = 11, +Se: n = 11) in B, (−Se: n = 8, +Se: n = 12) in C. Significance was defined as a p value of <0.05.