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. 2017 Mar 6;7:43674. doi: 10.1038/srep43674

Figure 4. Study species hybridized with the CAL32L probe targeting the calcibacterium.

Figure 4

(a,b) Filters containing isolated calcibacteria from Hemimycale columella (EM). (c) Filters containing isolated calcibacteria from Cliona viridis (EM). (d) H. columella choanosome harbouring larvae (CM). (e) Tissue section of Cinachyrella alloclada (EM). (f) Ectosome of H. columella whitish morph (CM); note that most calcibacteria are released into the sponge mesohyl forming dense aggregates. (g) Choanosome of H. columella whitish morph (CM); note that most calcibacteria are contained within calcibacteriocytes, surrounding the cell nucleus (arrowheads). (h) Ectosome section of H. columella, pinkish morph (CM). (i) Hybridized ectosome section of Protosuberites sp. (CM). Blue colour corresponds to sponge nuclei and bacteria nucleoid; green colour represents hybridization points; reddish colour results from self-fluorescence of cyanobacteria and pinkish colour results from overlapping DAPI blue-stained nuclei and reddish self-fluorescence of cyanobacteria. EM, Epifluorescence Microscopy; CM, Confocal Microscopy.