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. 2017 Mar 6;7:43592. doi: 10.1038/srep43592

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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ChIP assays were used to examine di-methylated H3K9 (A), tri-methylated H3K27 (B), tri-methylated H3K4 (C), SETDB1 (D), Ezh2 (E) and MLL2 (F) occupancy in three regions of the IL-6 promoter in normal and OA SF. The results were normalized to the percentage in the input control. Data are shown as the means ± SD. All of the data were obtained from 20 OA patients receiving knee-replacement surgery and 15 samples of non-arthritic synovial tissues obtained during arthroscopy.