Skip to main content
. 2017 Mar 6;7:43575. doi: 10.1038/srep43575

Figure 2. Activation of SREBP-2 and expression of HMG-CoA reductase.

Figure 2

(A) Proteolytic activation of SREBP-2 was analyzed by Western blotting. The cells were grown in the presence of 10% FCS or set to serum-free medium for 16 h before measurements. Upper panels: representative blots. Lower panels: Quantification showing means ± SEM. (B) mRNA expression of HMG-CoA reductase was analyzed by quantitative PCR. Shown are means ± SEM of n = 3 (10% FCS) or n = 5 (16 h serum-free medium) independent experiments. (C) HMG-CoA reductase protein expression was analyzed by Western Blotting. The cells were grown in the presence of 10% FCS or kept for 16 h in serum-free medium. Upper panels: representative blots. Lower panels: Quantification showing means ± SEM. (D) Western blot analysis of phospho-AMPKα (Thr172) and AMPKα in MEFs grown in the presence of 10% FCS or in serum-free medium supplemented with growth factors (representative blot). *p < 0.05; **p < 0.01; n.s., not significant in one-sample t-test.