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. 2017 Mar 6;7:43570. doi: 10.1038/srep43570

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Bone-marrow-derived mast cells (BMMCs) exposed for 1 h to ENMs at 25 & 50 μg/ml were analyzed via flow cytometry for Annexin V/propidium iodide (PI) expression on the surface of apoptotic cells. Hydrogen peroxide (H₂O₂, 10 mM) and capsaicin (Cap, 100 μM) were used as positive controls (white bars). Horizontal dash line represents NT control values. Values are expressed as mean ± SEM (N = 3/group). *Indicates significant difference between non-treated control group (p ≤ 0.05).