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. 2017 Mar 6;7:43474. doi: 10.1038/srep43474

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative Hematoxylin and eosin-stained tissue sections of the intrascapular BAT depot from control (A) and alcohol consuming mice (B). Image analysis of BAT shows a significantly increased number of nuclei/field in alcohol fed mice (C). There was no significant difference in the number of lipid droplets/cell in control vs. alcohol-fed mice (D); however, alcohol-fed mice had a significantly smaller percentage of large lipid droplets, and a significantly higher percentage of small lipid droplets (E). The concentration of triglycerides in the BAT of alcohol-fed was significantly lower (F). Scale bars in A and B = 20 μm. (C–F) analyzed by Student’s t-test; *p < 0.05. Sample size: (C–E), all groups n = 3; (F) control and alcohol n = 5.