Figure 4. SF162 HIV-1 challenge of humanized mice treated with VRC01 mRNA-LNPs or VRC01 protein.
BLT humanized mice were i.v. injected with 30 μg of Luc mRNA-LNP (negative control), 600 μg (28 mg kg−1) VRC01 mAb or 30 μg (1.4 mg kg−1), 15 μg (0.7 mg kg−1), 7.5 μg (0.35 mg kg−1) of VRC01 mRNA-LNP. (a) Animals were bled 24 h post-injection and VRC01 mAb levels were measured in the plasma by ELISA. (b) Viral loads (RNA copies per 10 μl plasma) at 2 weeks post-challenge with the SF162 HIV-1 isolate were measured by qRT–PCR. Error bars are s.e.m. Statistics: (a) One-way analysis of variance with Bonferroni corrections P<0.001 in all marked categories, (b) Student's t-test, two-sided, comparing Luc treated to VRC01 treated were highly significant: P<0.001. Group size is five or six animals.