Figure 4. The rs7090445 risk allele disrupts RUNX3 binding.

(a) ENCODE ChIP-seq data from the rs7090445 heterozygote lymphoblastoid cell line GM12878. Mean read counts for each allele were combined from replicates, error bars show SEM. Meta P value calculated using Fisher’s method combining binomial test P values. (b) Electromobility shift assay (EMSA) in GM12878 showing differential nuclear protein binding to alleles of rs7090445. Increased protein binding observed for the non-risk T allele was shifted by the addition of a RUNX3 antibody. An uncropped EMSA gel image is provided in Supplementary Fig. 9.