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. 2017 Mar 6;7:43636. doi: 10.1038/srep43636

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(a) A nuclear enriched Kss1 allele is generated by the addition of two nuclear localization sequences upstream of the coding sequence of the proteins. The 2xNLS-Kss1-YFP is visualized by microscopy in cells bearing a histone tag CFP before (−3 min) and after (+5 min) α-factor addition at time 0. (b) Nuclear to cytoplasmic ratio of a WT and 2xNLS-Kss1 quantified during a time-lapse movie with addition of pheromone. WT: blue, Nc = 334; 2xNLS-Kss1: red, Nc = 677.