Figure 7. Clonally expanded CD27^lo/negVδ1⁺ T cells express CX₃CR1 and cytotoxic effector molecules.

(a) Expression of intracellular cytotoxic effector molecules, granzyme A, granzyme B and perforin by CD8⁺ T _Naïve, CD8⁺ T _EMRA, Vδ1⁺ CD27^lo/neg and Vδ1⁺ CD27^hi cells. Correlation graph between all 7 donors and the corresponding TCRδ D75 calculated from TCR repertoire deep sequencing. Representative histograms, graph and dot plot are from 7 donors. (b) Expression of CX₃CR1 on the surface of CD8⁺ T _Naïve, CD8⁺ T _EMRA, Vδ1⁺ CD27^lo/neg and Vδ1⁺ CD27^hi cells. Within CX₃CR1⁺ or CX₃CR1- Vδ1⁺ T cells the expression of granzyme B and IL7Rα. Representative histograms of 5 donors (left), with all 5 donors shown (middle) and 4 of these donors assessed for CX₃CR1/granzyme B/IL7Rα (right). (c) Single cell γδ TCR analysis from 3 donors, single cells were sorted from CX₃CR1⁺ or CX₃CR1- Vδ1⁺ T cells and CDR3 sequences analysed against dominant clonotypes identified by deep sequencing. Graphs show the mean±s.e.m. and data were analysed by two-way ANOVA (a) and one-way ANOVA with Holm-Sidak's post-tests (b), ***P<0.001 and ****P<0.0001.