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. 2017 Mar 6;7:43470. doi: 10.1038/srep43470

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative retinal cross-sections of control (CO) and ischemic (I/R) eyes stained using specific antibodies directed against the ECM glycoproteins fibronectin (A,B, red), laminin (D,E, green), tenascin-C (G,H, green) and tenascin-R (J,K, green) as well as the nuclear dye TO-PRO-3 (blue) shown as merged images. Small inserts display TO-PRO-3 (blue) and glycoprotein (green/red) staining separately. In both experimental groups, fibronectin as well as laminin displayed a blood vessel-associated staining. In addition, laminin was found in the ILM and in the GCL. Tenascin-C and -R staining was mainly localized in the IPL, OPL and GCL. Quantification revealed a significant increase in the fibronectin and tenascin-R staining area in ischemic retinae, whereas no significant changes were observed regarding the tenascin-C and laminin staining (C,F,I,L). Values are mean ± SEM ± SD. *p ≤ 0.05; **p ≤ 0.01; n = 5/group. GCL = ganglion cell layer, ILM = inner limiting membrane, INL = inner nuclear layer, IPL = inner plexiform layer, ONL = outer nuclear layer, OPL = outer plexiform layer. Scale bar = 50 μm.