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. 2017 Mar 6;7:43470. doi: 10.1038/srep43470

Table 1. List of primer pairs used for analyses of ECM glycoprotein and CSPG mRNA expression in control (CO) and ischemic (I/R) rat retinae and optic nerves by qRT-PCR.

Gene Primer sequence Amplicon size Primer efficiency (retina/optic nerve) Reference
β-Actin_for CCCGCGAGTACAACCTTCT 72 bp 1.000/− 52
β-Actin_rev CGTCATCCATGGCGAACT
Aggrecan_for TGGCTGCAGGACCAGACT 97 bp 1.000/1.000 This study
Aggrecan_rev CGCCATAGGTCCTGACTCC
Brevican_for AGCAGAACCGCTTCAATGTC 61 bp 0.825/0.838 This study
Brevican_rev TCAGAGAAGGCAGAGGGATG
Cyclophilin_for TGCTGGACCAAACACAAATG 88 bp −/0.939 52
Cyclophilin_rev CTTCCCAAAGACCACATGCT
Fibronectin_for CAGCCCCTGATTGGAGTC 73 bp 0.909/0.775 This study
Fibronectin_rev TGGGTGACACCTGAGTGAAC
α1-Laminin_for CCCTGGGATGAAGAAGCA 101 bp 0.894/0.752 This study
α1-Laminin_rev CAGGGCTGCTGATGGAAG
RPTPβ/ζ CA_for AACCATCCTTGGAAAACACG 66 bp 1.000/0.757 52
RPTPβ/ζ CA_rev CATTGGTGAGATTTATTTCCACTGT
RPTPβ/ζ PTP1_for CCTCGTGGAGAAAGGAAGAAG 77 bp 0.953/0.739 52
RPTPβ/ζ PTP1_rev CCAGGAAGCTCCCGTATTCT
Tenascin-C_for GCTCTCCTATGGCATCAAGG 60 bp 0.805/1.000 52
Tenascin-C_rev TCATGTGTGAGGTCGATGGT
Tenascin-R_for TCATCTCCATTACTGCTGAGAGG 93 bp 0.769/0.779 This study
Tenascin-R_rev AGTGCAAGTGGGAGATAGGG

For relative quantification of mRNA levels in the retinae and optic nerves the genes β-actin and cyclophilin served as housekeeping genes, respectively. The primer sequence, the predicted amplicon size, primer efficiency for retinae and optic nerves and the reference are indicated. Abbreviations: bp = base pairs, for = forward, rev = reverse.