Table 3. Adjustments set for the ImageJ macro.
| Glycoprotein/CSPG | Tissue | Background subtraction (pixel) | Lower threshold | Upper threshold |
|---|---|---|---|---|
| Aggrecan | Retina | 50 | 16.21 | 85 |
| Optic nerve | 50 | 14.13 | 85 | |
| Brevican | Retina | 100 | 16.88 | 85 |
| Optic nerve | 50 | 20.40 | 85 | |
| Fibronectin | Retina | 50 | 16.39 | 85 |
| Optic nerve | 50 | 15.07 | 85 | |
| Laminin | Retina | 50 | 19.84 | 85 |
| Optic nerve | 50 | 12.92 | 85 | |
| Phosphacan/RPTPβ/ζlong (473HD antibody) | Retina | 50 | 7.35 | 85 |
| Optic nerve | 50 | 21.58 | 85 | |
| RPTPβ/ζ-isoforms (KAF13 antibody) | Retina | 50 | 11.58 | 85 |
| Optic nerve | 50 | 20.26 | 85 | |
| Tenascin-C (KAF14 antibody) | Retina | 50 | 9.47 | 85 |
| Optic nerve | 10 | 19.95 | 85 | |
| Tenascin-R | Retina | 50 | 22.43 | 85 |
| Optic nerve | 10 | 10.57 | 85 |
For area analyses of retinae and optic nerves, background subtraction as well as the lower and upper threshold was set for each stain as indicated.