Fig. 6.

Effect of p38, Erk 1/2 and PI3K pharmacological inhibitors and DNp85 mutant on G-CSF-induced β1 integrin expression. Monolayers of Swan 71 cells maintained 24 h in serum-free medium were (A) pre-treated for 1 h at 37 °C with or without SB (2 μM), PD (1 μM) or Ly (1 μM) or (B) transfected with DNp85 mutant or the corresponding control vector. Cells were then incubated for 4 h in the presence or absence of 100 ng/ml of G-CSF. Western blot assays were performed with anti-β1 integrin or anti-actin antibodies. Results from one representative experiment are shown (upper panel). Data quantification was performed by densitometric analysis (lower panel). Statistical analyses were performed by one-way ANOVA followed by Tukey's Multiple Comparison Test (A) or Student's t-test (B). * p<0.05, n=3.