Table 2.
Clones, vendors and dilution/concentration of monoclonal antibodies
| Antibody | Clone (species) | Vendor | Dilution | Concentration (μg/ml) | AR |
|---|---|---|---|---|---|
| Bcl-2 | 124 (mouse) | DAKO Carpentaria, CA | 1:250 | 0.60 | AR, pH 9 |
| CD3 | SP7 (rabbit) | ThermoFisher Fremont, CA | 1:20 | * | No AR, AR, pH 6 or 9 |
| 1:40 | * | ||||
| 1:100 | * | ||||
| CD20 Ab-1 | L26 (mouse) | ThermoFisher | 1:20 | * | No AR, AR, pH 6 or 9 |
| 1:40 | * | ||||
| 1:100 | * | ||||
| LCA (CD45) Ab-2 | 135-4C5 (mouse) | Neomarkers, Fremont, CA | 1:20 | 10 | No AR, AR, pH 6 or 9 |
| 1:40 | 5 | ||||
| 1:100 | 2 | ||||
| Cleaved caspase 3 | D175 (rabbit) | Cell Signaling, Danvers, MA | 1:200 | 0.5 | AR, pH 9 |
| Cytokeratins | AE1/AE3 (mouse) | Millipore Corp., Temecula, CA | 1:5000 | 0.2 | AR, pH 9 |
| E-cadherin | EP700Y (rabbit) | ThermoFisher | 1:20 | * | AR, pH 9 |
| 1:40 | * | ||||
| 1:200 | * | ||||
| 1:400 | * | ||||
| EGFr | 31G7 (mouse) | Zymed, San Francisco, CA | 1:3 | 5 | No AR |
| Estrogen Receptor alpha | 6F11 (mouse) | Santa Cruz Biotechnology, Santa Cruz, CA | 1:20 | * | AR, pH 9 |
| HER2 | Ab-3 (mouse) | Calbiochem, Cambridge, MA | 1:200 | 0.5 | AR, pH 9 |
| Ki67 | SP6 (rabbit) | ThermoFisher, | 1:1000 | * | AR, pH 9 |
| PCNA | PC-10 (mouse) | Santa Cruz Biotechnology | 1:15000 | 0.0067 | AR, pH 9 |
| Progesterone Receptor | PgR1294 (mouse) | DAKO | 1:200 | * | AR, pH 9 |
*
Concentration not specified by vendor.