Figure 5.

(A) Effect of PEA (1–5 μM) on intracellular Ca²⁺ elevation by 2‐AG in HEK‐293 cells over‐expressing human TRPV1 channels. PEA was pre‐incubated with cells 5 min prior to 2‐AG treatment. (B) Effect of PEA (1–5 μM) on 2‐AG‐induced TRPV1 channel desensitization of the effect of capsaicin (0.1 μM) in HEK‐293 cells over‐expressing human TRPV1 channels. PEA was pre‐incubated with cells 5 min prior to 2‐AG treatment. The effect on intracellular Ca²⁺ of capsaicin (0.1 μM) alone were set as 100%. Data are means ± SE of n = 4 separate determinations. Dose–response curves were fitted by a sigmoidal regression with variable slope. Curve fitting and parameter estimation were performed with GraphPad Prism.